Image Lab Software is an image acquisition and analysis program that allows you to analyze gel or blot features, capture optimized image data and generate a comprehensive report in just a few seconds. It runs on the following Bio-Rad imaging systems: - Gel Doc EZ imager and Gel Doc XR - ChemiDoc MP and ChemiDoc XRS - GS-900 calibrated densitometer. To install Image Lab on a Mac. Insert the Image Lab software CD in your CD-ROM drive. Double-click the CD icon on your desktop to see the folder contents. Double-click the file Image Lab.dmg. Drag the Image Lab application icon into the Applications folder. Follow the instructions in Connecting the Imager to the Computer to connect your. Image Lab software is for personal computers running Windows and Mac OS and is a powerful yet easy to use package for acquisition and analysis of gel and blot images. Image Lab features simplified lane loading normalization and automated detection of lanes and bands with complete report generation. Image Lab standard edition can be downloaded free. Download the Image Lab Software 6.0. It is able to open jpg and tiff files. I just spoke to the Bio-rad representative who gave us a training on our new Chemidoc MP System!

One important factor to consider is what file types the software accepts, says John Wiktorowicz, a professor at the University of Texas Medical Branch at Galveston. Some use a proprietary format linked to the company’s imaging system, while many accept generic formats such as JPEG or BMP. Wiktorowicz, director of proteomics for the biomolecular resource facility at the university, prefers to work with TIFFs. “It leaves the values intact,” he explains. Other formats such as JPEG compress the file size and could result in loss of crucial information. “I would never use JPEGs for any quantitative study,” says Wiktorowicz.

Any imaging software should save raw data files, and record any modifications users make to an image. “When it comes time to publish, you need that information,” says Mark Chen, a graduate student at Duke University.

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For certain users, such as those who produce pharmaceuticals, a complete data trail is required by the Food and Drug Administration, as laid down in the Code of Federal Regulations Title 21, Part 11 (CFR 21 Part 11). This is particularly important for drug production and quality control, and some clinical labs may want this added layer of record keeping and password security, says Raymond Miller, a product manager at Bio-Rad in Hercules, California.

The simplest choice, Chen says, is to work with the software that comes with your imager. Internet account. For researchers who want more options, The Scientist profiles five imaging programs.

IMAGEJ
imagej.net/Welcome

The public domain ImageJ software platform, developed at the National Institutes of Health and augmented by various users, is loved by some and hated by others. ImageJ fan Corentin Cras-Méneur, an assistant professor at the University of Michigan Medical School in Ann Arbor, appreciates the flexibility. “It’s one shop for everything,” he says. “With ImageJ I would analyze Western blots, I would do some quantifications of fluorescent microscopy, I would control the microscope . . . anything you can think of.”

For most users, standard ImageJ should be sufficient to analyze bands on a gel or Western, Cras-Méneur says. But for those who want more, many plug-ins are available; coders versed in the Java language can also create their own plug-ins or macros. For example, Cras-Méneur uses a plug-in that analyzes how background signal varies across an image and subtracts it from bands accordingly, instead of assuming the background is uniform.
PROS

With a variety of plug-ins available, ImageJ is flexible.
It supports stacked images, such as pictures collected from a series of different levels using a confocal microscope.

CONS

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0007ImageJ wasn’t specifically designed for gel or blot analysis, and can be intimidating to new users. While many common questions are answered in online forums, there’s no tech support line to call if you have a specific query. “It’s a lot easier if you have someone around you who has been using it,” says Cras-Méneur.
Letitia Jones, a postdoc at the University of Rochester Medical Center in New York, disliked the fact that ImageJ typically uses a single box size for all bands on a gel, even if some bands stretched or smiled. She found that, depending on the box drawn by individual users, the results varied quite a bit. “Two people can have totally different conclusions,” says Jones. She prefers Image Studio (see below) because it allows her to customize the box to each band.
0007Juan Pablo de Rivero Vaccari, an assistant professor at the University of Miami Miller School of Medicine, says ImageJ’s results didn’t match what he saw with his own eyes—he could tell a band was darker, but the numbers coming out of the software didn’t back him up. He switched to another program, UN-SCAN-IT gel (see below).

IMAGE STUDIO
www.licor.com/bio/products/software/image_studio_lite

SOME, MORE, MOST: All programs discussed in this article can quantify band weight. LI-COR’s Image Studio shown here.COURTESY OF LI-COR BIOSCIENCESThe Image Studio software comes with imaging instruments from LI-COR Biosciences, but any scientist can download the Lite version. It’s the same software, minus the ability to control imagers.

Most users are happy with the Lite version, notes Jeff Harford, senior product marketing manager at LI-COR in Lincoln, Nebraska. However, LI-COR also sells additional, optional features. These include analysis of two-color or in-cell Westerns; a small-animal imaging tool that allows users to draw shapes around objects such as tumors or organs; and a tool to analyze multiwell plates.
PROS

Chen says the design reminds him of Microsoft Word or Excel, making Image Studio easy to pick up.
You can sort your files based on parameters such as image date, the type of analysis, or the fluorescent color channels you used. “It’s easy to find old images,” says Chen.
You can customize the box around each band, to fit bands that stretched or smiled.

CONS

The software is primarily focused on a few types of analysis, notes Harford. Scientists who want other functions, such as colony counting or microscopy image analysis, could do it with Image Studio but may prefer to look for software dedicated to their needs.
0007Jones notes that many tutorials are video format, while she has a harder time finding the written instructions when she wants them. H

Bio Rad Image Lab Download Mac